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Epoxide hydrolase activity in human skin.
Author(s) -
O'Neill VA,
Rawlins MD,
Chapman PH
Publication year - 1981
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1981.tb01260.x
Subject(s) - epoxide hydrolase , microsome , epoxide , dermis , chemistry , human skin , microsomal epoxide hydrolase , epidermis (zoology) , substrate (aquarium) , styrene oxide , biochemistry , chromatography , enzyme , biology , styrene , organic chemistry , anatomy , ecology , genetics , polymer , copolymer , catalysis
1 Epoxide hydrolase (EH) activity was measured in biopsied skin (n = 42) using 7‐[H3]‐styrene oxide as substrate, and separation of the products by high performance liquid chromatography. 2 EH activity (mean +/‐ s.d.) was present in separated epidermis (139 +/‐ 105 pmol glycol formed mg‐1 min‐1) and dermis (165 +/‐ 120 pmol glycol formed mg‐1 microsomal protein min‐1). 3 Whole skin EH activity (mean +/‐ s.d.) varied widely (433 +/‐ 254 pmol glycol formed mg‐1 microsomal protein min‐1) 4 No significant difference in EH activity was observed in skin from breast, penis and leg. 5 Skin EH activity does not appear to contribute significantly to the systemic metabolism of epoxide, but may be important in determining the effects of epoxides formed within the epidermis.