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Radioimmunoassay for trifluoperazine in human plasma.
Author(s) -
Midha KK,
Hubbard JW,
Cooper JK,
Hawes EM,
Fournier S,
Yeung P
Publication year - 1981
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1981.tb01199.x
Subject(s) - radioimmunoassay , trifluoperazine , pharmacokinetics , antiserum , bioavailability , drug , pharmacology , chemistry , chromatography , plasma concentration , medicine , immunology , antibody , biochemistry , enzyme , calmodulin
1 A new sensitive and rapid radioimmunoassay procedure for the determination of the plasma concentrations of the neuroleptic drug trifluoperazine is described. 2 The antiserum developed for trifluoperazine cross‐reacted with N‐desmethyltrifluoperazine and 7‐ hydroxytrifluoperazine to the extent of 26 and 24% respectively but its cross‐reactivity with commonly co‐administered tricyclic antidepressants and antianxiety agents tested was negligible. 3 The assay, based on the above antiserum, enabled the quantitation of 50 pg of the drug in 200 microliters of plasma with a coefficient of variation of about 2% and therefore should be applicable for singly dose pharmacokinetic and bioavailability studies. It should be applicable to therapeutic monitoring of the drug in patients.