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Monooxygenase activity of human liver in microsomal fractions of needle biopsy specimens.
Author(s) -
Boobis AR,
Brodie MJ,
Kahn GC,
Fletcher DR,
Saunders JH,
Davies DS
Publication year - 1980
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1980.tb04790.x
Subject(s) - microsome , cytochrome , biopsy , liver biopsy , medicine , chemistry , pathology , endocrinology , biochemistry , enzyme
1 Methods are described for the determination of mixed function oxidase activity in microsomal fractions from percutaneous needle biopsies of human liver. 2 Activities of needle biopsy samples were comparable with those of wedge biopsy sample obtained at laparotomy from different subjects. 3 Although cytochrome P‐450 content of liver from rat and man was similar, human AHH activity was only 10% of that in the rat. 4 In biopsies with preserved hepatic architecture, AHH activity and cytochrome P‐450 content showed a significant positive correlation. 5 Cigarette smoking significantly increased both AHH activity and turnover number, but not cytochrome P‐450 content, of biopsies with normal architecture. 6 The presence of liver disease caused a significant decrease in AHH activity and cytochrome P‐450 content.