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Specific radioimmunoassay of amitriptyline and nortriptyline.
Author(s) -
Brunswick DJ,
Needelman B,
Mendels J
Publication year - 1979
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1979.tb00943.x
Subject(s) - nortriptyline , amitriptyline , radioimmunoassay , chromatography , chemistry , pharmacology , tricyclic , medicine , biochemistry
1 Antisera to nortriptyline were prepared by immunizing rabbits with N‐ succinylnortriptyline‐bovine serum albumin conjugate. 2 A sensitive radioimmunoassay has been developed for the tricyclic antidepressants amitriptyline and nortriptyline. 3 amitriptyline and nortriptyline are separated from each other and from interfering metabolites before assay. 4 Using [3H]‐imipramine and [3H]‐succinylnortriptyline as tracers the radioimmunoassay can measure amitriptyline and nortriptyline levels down to 2–3 ng/ml using 0.05 ml plasma sample. 5 Agreement between the radioimmunoassay and a gas‐chromatographic assay was excellent for both amitriptyline and nortriptyline.