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The application of HPLC to the determination of some 1,4 benzodiazepines and their metabolites in plasma.
Author(s) -
Skellern GG,
Meier J,
Knight BI,
Whiting B
Publication year - 1978
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1978.tb01661.x
Subject(s) - chromatography , high performance liquid chromatography , pharmacology , plasma concentration , chemistry , metabolite , pharmacokinetics , medicine , biochemistry
1 A high pressure liquid chromatographic (HPLC) assay has been developed for the measurement of chlordiazepoxide and its metabolites, N‐desmethylchlordiazepoxide and demoxepam in microgram concentrations in human plasma. 2 Another metabolite was detected in appreciable amounts in the plasma from patients receiving high doses of chlordiazepoxide after the first 3 days of treatment. 3 Preliminary studies would indicate that this metabolite is N‐desmethyldiazepam. 4 A modified HPLC system, using a microparticulate column was developed to separate N‐desmethyldiazepam from chlordiazepoxide and its other metabolites in plasma.