Premium
A spectrofluorometric method for the determination of ajmaline in plasma.
Author(s) -
Welman E,
Curry PV,
Krikler DM,
Rowlands E,
Callowhill EA
Publication year - 1977
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1977.tb00784.x
Subject(s) - ajmaline , chemistry , fluorescence , excitation wavelength , fluorescence spectroscopy , plasma , chromatography , medicine , physics , quantum mechanics
1 Ajmaline was found to have maximum fluorescence at neutral pH with 300 nm excitation and 365 nm emission wavelengths (corrected). 2 The fluorescence intensity had a linear relationship to concentration up to 50 microgram ml‐1 and the recovery of ajmaline after extraction from plasma was 92.5 +/‐ 3%. 3 Extraction of drug‐free plasma and of samples containing known concentrations of ajmaline showed that drug levels in the range found clinically could be measured accurately by fluorimetry. 4 Serial plasma ajmaline concentrations were measured in a subject after intravenous injection of ajmaline (50 mg). The rates of plasma clearance of the drug were found to be similar to those obtained in previous studies.