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Rapid fluorimetric assay of minocycline in plasma or serum: comparison with microbiological assay.
Author(s) -
Hall D
Publication year - 1977
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1977.tb00667.x
Subject(s) - minocycline , chromatography , chemistry , agar , pharmacokinetics , antibacterial agent , pharmacology , antibiotics , medicine , biology , bacteria , biochemistry , genetics
1. Plasma concentrations of minocycline were assayed by measuring the fluorescence emission of an aluminum‐minocycline chelate in a spectrofluorimeter. 2. The assay gives reproducible results over the concentration range 0.05‐5.0 mug ml‐1, and compared favourably with results achieved using a conventional large plate agar diffusion microbiological assay in a double‐blind comparison of the two methods. 3. Fluorimetric assay of minocycline in plasma was found to correlate closely with antibiotic activity (r = 0.95). The method is easy and economical to perform, results being obtained in 1 h.