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SOME CLINICAL IMPLICATIONS OF THE PROTEIN BINDING OF DIGOXIN
Author(s) -
WALLACE SUSAN,
WHITING B.
Publication year - 1974
Publication title -
british journal of clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.216
H-Index - 146
eISSN - 1365-2125
pISSN - 0306-5251
DOI - 10.1111/j.1365-2125.1974.tb00261.x
Subject(s) - digoxin , ultrafiltration (renal) , plasma protein binding , blood proteins , albumin , chemistry , serum albumin , endocrinology , medicine , pharmacology , chromatography , biochemistry , heart failure
1 The binding of [ 3 H]‐digoxiri to human plasma proteins was studied using both ultrafiltration and frontal analysis, to establish a suitable method for the measurement of digoxin‐protein interaction, and to assess the effect of variations in digoxin protein binding on its distribution. 2 Using ultrafiltration, [ 3 H]‐digoxin was found to bind to plasma proteins to the extent of 30–40% over a concentration range of 0.001‐1 ng/ml. Frontal analysis was unsuccessful in the measurement of digoxin protein binding. 3 Dilution experiments using plasma and human serum albumin suggested that variations in digoxin protein binding would be unlikely to cause significant alterations in the levels of free drug in the body. 4 The possible clinical implications of these results are discussed.