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Co‐expression and regulation of ovarian vitellogenins in the P acific oyster C rassostrea gigas
Author(s) -
LleraHerrera Raúl,
VázquezBoucard Celia,
GarcíaGasca Alejandra,
Huvet Arnaud
Publication year - 2014
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2012.03246.x
Subject(s) - vitellogenin , vitellogenins , biology , vitellogenesis , crassostrea , oyster , medicine , yolk , endocrinology , gene expression , zoology , gene , genetics , ecology , oocyte , embryo
In addition to the reported single c DNA sequence encoding vitellogenin in the P acific oyster C rassostrea gigas (referred as Cg ‐Vtg1), we isolated and characterized the 3′ end of a novel vitellogenin (Vtg) in C . gigas , named Cg ‐Vtg2. The transcript size is up to 6 kb long and includes a D ‐type von W illebrand domain, characteristic of most vitellogenins, but not occurring in Cg ‐Vtg1. The expression of Cg ‐Vtg2 was found restricted to follicular cells in maturing and ripe female gonads. Quantitative expression analysis of both vitellogenins showed a closely correlated transcription pattern during the female gametogenic cycle, with the highest expression during the ripe (mature) stage, and a declining expression, to almost undetectable levels, in post‐spawning (resting) stage. Levels of vitellogenin m RNA associates with the quantity of ingested food. Mature female oysters fed either a 2% or a 12% algal biomass per female biomass, showed a 2.9‐fold increase in expression of Cg ‐Vtg1 and a 3.7‐fold increase of Cg ‐Vtg2 expression levels in those females fed the higher level of algae, suggesting that Vtg's could be used as quantitative indicator of vitellogenesis. Our results suggest that vitellogenins are synthesized during the late period of vitellogenesis, when most of the yolk has already been incorporated into maturing oocytes.