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Fertilization by refrigerator stored sperm of the Indian major carp, L abeo calbasu ( H amilton, 1822)
Author(s) -
Hassan Md Mahbubul,
Nahiduzzaman Md,
Al Mamun Shaheed Nasrullah,
Taher Md Abu,
Hossain Mostafa Ali Reza
Publication year - 2013
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2012.03214.x
Subject(s) - biology , sperm , human fertilization , carp , hatching , artificial fertilization , sperm motility , extender , zoology , andrology , fishery , botany , anatomy , fish <actinopterygii> , chemistry , medicine , organic chemistry , polyurethane
This study reports on the spermatological properties, and on the development of a protocol for refrigerator storage (4°C) of L abeo calbasu ( H amilton, 1822) sperm for artificial breeding. Volume, motility, concentration and pH of the freshly collected sperm were 2.21 ± 0.53 (μL g −1 of fish weight) (mean ± SD ), 95 ± 1 (%), 1.93 ± 0.44 × 10 9 (cells mL −1 ) and 7.56 ± 0.17 respectively. Sperm activation was evaluated at different osmolalities of NaCl solution, and motility ceased completely when osmolality of the extender was ≥287 mOsmol kg −1 . Sperm retained motility for 24, 72 and 108 h, after refrigerator storage when sperm were undiluted, suspended in A lsever's solution and suspended in A lsever's solution containing 5% methanol respectively. Fertilization rate of fresh eggs with sperm stored at 4°C in A lsever's solution and A lsever's solution containing 5% methanol was 77% and 60% with a hatching rate of 60% and 43% respectively. The fertilization and hatching success of the stored sperm suggests potential to use refrigeration for transporting genetic material to hatcheries for artificial breeding of L . calbasu in B angladesh.