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Inhibitory activity of essential oils from medicinal plants against P seudomonas sp. isolated from aquatic environments
Author(s) -
Vaseeharan Baskaralingam,
Sivalingam Manju,
Palaniappan Ramasamy
Publication year - 2013
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2012.03208.x
Subject(s) - chloroform , pseudomonas aeruginosa , antimicrobial , biology , minimum inhibitory concentration , agar diffusion test , agar , pseudomonas , chromatography , antibacterial activity , bacteria , microbiology and biotechnology , food science , traditional medicine , chemistry , medicine , genetics
This study focuses on the evaluation of the antimicrobial activity of essential oils ( EO s) from medicinal plants against P seudomonas sp isolated from aquatic environments, and proves its controlling efficacy on P seudomonas a eruginosa‐Dahp1 using Confocal Laser scanning Microscopy. Twenty‐seven P seudomonas spp. ( Ps 1 – Ps 27 ) were isolated using King's B medium and the selected P . aeruginosa‐Dahp1 was confirmed using 16S r‐ DNA methods. Antimicrobial activity of 10 EO s was determined using agar well and disc diffusion methods. Ten EO s were extracted with six solvents and the crude extracts were tested against the P seudomonas spp. through disc diffusion method. Among 10 EO s tested, maximum inhibitory activity was noted in EO s of W ringtia tinctoria against the P . aeruginosa‐Dahp1 in the disc diffusion method. The MIC values (concentrations were expressed in Weight/Volume) of the EO s range from 0.5 to 9.054 mg mL −1 . The EO of W . tinctoria showed the maximum activity at the concentration (w/v) of 2.060 mg mL −1 . The EO of W . tinctoria with chloroform extract showed the maximum inhibition against P . aeruginosa‐Dahp1 . The CLSM proves the control and viability of 1 × 10 5 CFU mL −1 of P . aeruginosa‐Dahp1 at a lower concentration (2.720 mg) of EO s with chloroform extracts of W . tinctoria . This study pivots for designing of new drugs using EO s of W . tinctoria against P seudomonades in the aquaculture sites.