Premium
Studies on quantitative trait loci related to superoxide dismutase in mirror carp ( C yprinus carpio L .)
Author(s) -
Xu Yulan,
Zhang Xiaofeng,
Zheng Xianhu,
Kuang Youyi,
Lu Cuiyun,
Cao Dingchen,
Yin Sen,
Li Chao,
Sun Xiaowen
Publication year - 2013
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2012.03191.x
Subject(s) - quantitative trait locus , biology , cyprinus , microsatellite , genetics , superoxide dismutase , common carp , genetic linkage , carp , gene , allele , biochemistry , enzyme , fish <actinopterygii> , fishery
The F 1 progeny from parents selected randomly for differences in performance traits from different families of mirror carp were used as a mapping panel. Linkage maps constructed using J oin M ap 4.0 software with 992 microsatellite polymorphic markers ultimately yielded 51 groups. Composite interval mapping ( CIM ) of superoxide dismutase ( SOD ) trait of three tissues (liver, anterior and posterior intestine) was conducted for quantitative trait loci ( QTL ) location using M ap QTL 6.0 software. Results shows that seven QTL s significantly ( P < 0.05) explained 8.2–18.5% of the phenotypic variations in liver, five QTL s explained 7.9–11.1% of the variation in anterior intestine ( P < 0.05) and six QTL s were located and explained 6.1–15.8% of the phenotypic variations in posterior intestine ( P < 0.05). NCBI BLAST n was used for homology analysis between the sequences from markers used in the located QTL regions and sequences from the zebrafish database, and many protein relating with the SOD expression were blasted (similarity >85%). These results showed that it is highly probable that these QTL s are the main regions of SOD expression. The data also support the value of forward genetic studies on organism development and serve as a basis for selection.