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Cryopreservation of summer flounder, P aralichthys dentatus L ., sperm
Author(s) -
Brown Ryan T,
Colburn Heidi R,
Nardi George C,
Berlinsky David L
Publication year - 2013
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2012.03163.x
Subject(s) - cryoprotectant , milt , cryopreservation , sperm , biology , extender , flounder , human fertilization , andrology , zoology , anatomy , botany , fishery , fish <actinopterygii> , chemistry , embryo , medicine , organic chemistry , polyurethane
The summer flounder, P aralichthys dentatus L ., is a high‐value species and considerable research has been conducted to determine practices conducive for its culture. As milt can be limited in this species, experiments were conducted to develop a practical sperm cryopreservation protocol for hatchery use. Two dilution ratios (1:2 and 1:4; sperm:extender), 2 diluents (saline and sucrose‐based), 2 cryoprotectants (10% DMSO and 12% glycerol) and 3 freezing rates (−5, −10 and −15°C min −1 ) were evaluated using differential staining to assess post‐thaw sperm survival. Seven combinations of the factors examined reduced post‐thaw viability by less than 30%. The average viability of sperm from fresh, pooled flounder milt (67.2 ± 2.9%) was not different from that of thawed milt diluted 1:4 with sucrose diluent (10% DMSO ) frozen at −5°C min −1 (38.4 ± 7.7%) and fertilization and hatch success were not different in trials using fresh or thawed, cryopreserved sperm. From these experiments a practical sperm cryopreservation method was developed, but further refinement of the freezing protocol is necessary to optimize results.