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Microsatellite–centromere mapping in bighead carp ( A ristichthys nobilis ) using gynogenetic diploid families
Author(s) -
Liu Lusha,
Tong Jingou,
Guo Wenjie,
Yu Xiaomu
Publication year - 2013
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2012.03155.x
Subject(s) - centromere , biology , genetics , ploidy , microsatellite , bighead carp , mendelian inheritance , meiosis , chromosome , allele , gene , carp , fish <actinopterygii> , fishery
Gene–centromere mapping using half‐tetrad analysis is a powerful tool for understanding chromosomal behaviour and determining the position of centromeres in relation to genes or markers in fish. In this study, eight gynogenetic diploid families induced by inhibiting of the second meiotic division were genotyped at 66 microsatellite loci for microsatellite–centromere ( M‐C ) mapping in bighead carp ( A ristichthys nobilis ). The absence of paternal alleles verified the success of gynogenetic development in all gynogenetic families. All loci were consistent with M endelian segregation in control families. The estimated recombination frequency ( y ) ranged from 0.057 to 0.875 with an average of 0.477 ± 0.222. Seventeen loci (25.76%) showed high M‐C recombination frequencies of over 0.667. M‐C distances ranged from 2.85 to 43.75 cM under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes, while mainly in the intermediate region. Information on centromere mapping could serve as a starting point to consolidate the genetic linkage groups in bighead carp.