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Bioassay‐guided isolation and identification of active compounds from M acleaya microcarpa (Maxim) Fedde against fish pathogenic bacteria
Author(s) -
Kang YuJun,
Yi Yanglei,
Zhang Chao,
Wu ShuQin,
Shi CunBin,
Wang GaoXue
Publication year - 2013
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2012.03123.x
Subject(s) - aeromonas hydrophila , vibrio anguillarum , biology , vibrio harveyi , microbiology and biotechnology , streptococcus iniae , pathogenic bacteria , bioassay , aeromonas salmonicida , minimum inhibitory concentration , bacteria , vibrio parahaemolyticus , vibrio , antimicrobial , genetics
Four alkaloids (Sanguinarine, 6‐Methoxyl‐dihydro‐chelerythrine, Cryptopine and β‐Allocryptopine) were isolated from aerial parts of M acleaya microcarpa (Maxim) Fedde using bioassay‐guided isolation method, and the inhibitory activity of ethanolic extract, various fractions and these four alkaloids against four fish pathogenic bacteria ( A eromonas hydrophila , A eromonas salmonicida , V ibrio anguillarum and V ibrio harveyi ) was assessed in vitro using the agar dilution method and the microdilution assay method respectively. A . hydrophila was the most sensitive strain to all the tested compounds. Minimum inhibitory concentration ( MIC ) values were lower for sanguinarine against all tested Gram‐negative strains than other three alkaloids, with MIC values of 12.5 mg L −1 for A. hydrophila and 50 mg L −1 to other pathogenic bacteria. Followed by 6‐methoxyl‐dihydro‐chelerythrine, which showed considerable antibacterial activity with MIC values of 80 mg L −1 for A . hydrophila , 100 mg L −1 for V . harveyi , and 125 mg L −1 for both V . anguillarum and A . salmonicida . Cryptopine and β‐allocryptopine revealed similar inhibitory activity with MIC values of 100 mg L −1 for A . hydrophila and 200 mg L −1 for other three bacterial species. These finding provided evidence that extract, as well as isolated compounds from M . microcarpa might be potential sources novel antibacterial agents for the treatment of fish infectious diseases.