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Silencing Pacific white shrimp Litopenaeus vannamei LvRab7 reduces mortality in brooders challenged with white spot syndrome virus
Author(s) -
Píndaro ÁlvarezRuiz,
Humberto MejíaRuiz Claudio,
Javier MagallónBarajas Francisco,
Marcial EscobedoBonilla César
Publication year - 2013
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2011.03084.x
Subject(s) - white spot syndrome , litopenaeus , biology , shrimp , penaeus monodon , rna silencing , virology , virus , prophenoloxidase , gene silencing , penaeus , rna interference , fishery , rna , gene , immunology , genetics , immune system , innate immune system
White spot syndrome virus ( WSSV ) is a major threat for farmed shrimp worldwide. RNA interference ( RNA i) is the most recent tool against viral diseases. Rab7 silencing effectively inhibited virus infections in juvenile shrimp, but the antiviral effect in brooders remains unknown. This study found a homologue Penaeus monodon Rab7 gene in Litopenaeus vannamei brooders from Mexico. Sequence identity was >99% to a Thai LvRab7 sequence and >94% to Rab7 sequences from P. monodon or Marsupenaeus japonicus . Animals treated with a partial (494 bp) or a complete (618 bp) LvRab7 ds RNA sequences and challenged 48 h post treatment (hpt) with a high WSSV dose showed 80–88% mortality respectively. Shrimp treated with 4 or 20 μg LvRab7 ds RNA and challenged with a WSSV high dose had 80% mortality each, but it was reduced to 33% and 40%, respectively, with a low dose. Efficacy of ds RNA to reduce shrimp mortality was dependent on virus dose used regardless of ds RNA concentration. A significant reduction in LvRab7 m RNA levels was observed at 120 hpt. In conclusion, silencing LvRab7 in brooders showed a mild antiviral effect against a WSSV challenge at 48 hpt.