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Isolation and characterization of bacteria associated with a syndrome disease of sea urchin S trongylocentrotus intermedius in N orth C hina
Author(s) -
Wang Yinan,
Feng Nisha,
Li Qiang,
Ding Jun,
Zhan Yaoyao,
Chang Yaqing
Publication year - 2013
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2011.03073.x
Subject(s) - biology , sea urchin , isolation (microbiology) , zoology , china , bacteria , microbiology and biotechnology , fishery , ecology , genetics , archaeology , history
Sea urchin, S trongylocentrotus intermedius , transplanted from J apan in 1989, has been widely cultured along the coasts of L iaoning and S handong P rovinces and has become the dominant and most economically important maricultured species in N orth C hina. However, a lesion syndrome symptom of S . intermedius broke out frequently these years, showing lethargy in activities, blackish peristomial membrane and body well lesions, and brought about high mortality eventually. Six representative prominent bacterial strains were isolated from diseased sea urchin from S eptember 2009 to J anuary 2010. By means of API 20 N E and 16S rRNA gene sequences analysis, isolates were identified as S hewanella aquimarina, P seudoalteromonas tetraodonis, V ibrio shilonii , V . harveyi, V . fortis and V . splendidus . Bacterial challenge tests showed that their representative isolates were virulent to S . intermedius with LD 50 values ranging from 9.2 × 10 4 to 3.4 × 10 6 CFU/g body weight, among which S . aquimarina , V. fortis and P. tetraodonis were highly virulent, and the other three isolates showed moderate virulence. The results indicated that a variety of bacteria including S hewanella , P seudoalteromonas and V ibrio were involved in the mortality of S . intermedius , and the six isolates were opportunistic pathogens of sea urchins. All isolates reported herein were sensitive to ampicillin, enrofloxacin, ofloxacin, doxycycline and florfenicol.