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In vitro comparisons of the inhibitory activity of florfenicol, copper sulphate and potassium permanganate towards Aeromonas hydrophila and Flavobacterium columnare
Author(s) -
Schrader Kevin K.,
Harries Marcuslene D.,
Darwish Ahmed M.
Publication year - 2013
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2011.03024.x
Subject(s) - aeromonas hydrophila , florfenicol , biology , microbiology and biotechnology , potassium permanganate , veterinary medicine , chemistry , fishery , fish <actinopterygii> , antibiotics , medicine , organic chemistry
Aeromonas hydrophila and Flavobacterium columnare , the aetiological agents of motile aeromonas septicaemia ( MAS ) and columnaris disease respectively, have been recently causing crippling mortalities to the sunshine bass, Morone chrysops female × Morone saxatilis male (Percichthyidae), industry in the United States. Isolates of A. hydrophila and F. columnare obtained from fish that died during farm outbreaks were subjected to in vitro evaluation of florfenicol ( FFC ), copper sulphate (Cu SO 4 ) and potassium permanganate ( KM nO 4 ). Florfenicol inhibited the growth of A. hydrophila and F. columnare more than Cu SO 4 and KM nO 4 . The minimum inhibition concentration ( MIC ) of FFC was 0.04 ± 0 and 0.2 ± 0.1 mg L −1 for A. hydrophila and F. columnare respectively, while the 50% inhibition concentration ( IC 50) for A. hydrophila and F. columnare was 0.23 ± 0.01 and 0.4 ± 0.2 mg L −1 respectively. Copper sulphate was more effective against A. hydrophila than KM nO 4 ; Cu SO 4 had a MIC of 83.2 ± 0 mg L −1 compared to 158.0 ± 0 mg L −1 for KM nO 4 . Copper sulphate was also more effective against F. columnare than KM nO 4 . The IC 50 values of Cu SO 4 and KM nO 4 towards F. columnare were 4.8 ± 0.3 and 8.7 ± 1.6 mg L −1 respectively, and the minimum bactericidal concentration values of Cu SO 4 and KM nO 4 towards F. columnare were 25.0 ± 0 and > 158.0 mg L −1 respectively. In addition, F. columnare was more sensitive to Cu SO 4 and KM nO 4 than A. hydrophila .