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Interactive effects of salinity on oxygen consumption, ammonium excretion, osmoregulation and Na + /K + ‐ ATP ase expression in the bullseye puffer ( Sphoeroides annulatus , J enyns 1842)
Author(s) -
PérezRobles Javier,
Re Ana Denisse,
GiffardMena Ivone,
Díaz Fernando
Publication year - 2012
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2011.02940.x
Subject(s) - osmoregulation , biology , ammonium , salinity , gill , excretion , zoology , atpase , oxygen , euryhaline , endocrinology , biochemistry , ecology , enzyme , fishery , fish <actinopterygii> , chemistry , organic chemistry
A physiological study was performed to explore the effects in Sphoeroides annulatus ( J enyns 1842) juveniles of exposure to different salinities 10, 17, 23, 29, 35 and 41‰ on oxygen consumption rate ( OCR ), ammonium excretion rate ( AER ), the oxygen‐nitrogen atomic ratio (O:N), osmoregulation and Na + /K + ‐ ATP ase expression. The OCR values ranged from 27.9 to 30.9 mg O 2 h −1 kg −1 , displaying a Type 1 response pattern Kinne (1977). AER ranged from 0.60 to 0.69 mgNH 4 +h −1 kg −1 , and O:N values were from 53.12 to 59.26, indicating that the puffers use proteins and lipids as an energy‐substrate. Osmoregulation in S. annulatus was hyposmotic in salinities of 23, 29, 35 and 41‰ and hyperosmotic in a salinity of 10‰. The isosmotic point of the bullseye puffer was 356 mmol kg −1 (10.5‰). The enzyme expression analysis indicates that the fish acclimated to 41‰ had the highest Na + /K + ‐ ATP ase expression level, whereas, the lowest expression level was found close to the isosmotic point. Na + /K + ‐ ATP ase expression in the gills was found to have a U‐shaped relationship with environmental salinity. We conclude that the bullseye puffer is strongly euryhaline and can be cultivated in a wide range of environments that has important economic implications.