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The parameters of energetic status of the wild Atlantic salmon ( Salmo salar L.) spermatozoa
Author(s) -
Dziewulska Katarzyna,
Rzemieniecki Adam,
BaranowskaBosiacka Irena,
Domagała Józef,
Chlubek Dariusz
Publication year - 2012
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2011.02833.x
Subject(s) - hypoxanthine , guanosine , biology , inosine monophosphate , inosine , energy charge , biochemistry , nucleotide , adenosine monophosphate , adenosine diphosphate , salmo , sperm motility , adenine nucleotide , nicotinamide adenine dinucleotide , adenosine , adenosine triphosphate , guanosine monophosphate , sperm , nad+ kinase , adenylate kinase , enzyme , fishery , platelet , botany , platelet aggregation , fish <actinopterygii> , immunology , gene
The energetic status of fresh sperm of wild Atlantic salmon ( Salmo salar L.) was presented in selected males ( n =10) with motility rate ≥80%. Purine and pyridine nucleotides: adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), guanosine triphosphate, guanosine diphosphate, guanosine monophosphate, inosine monophosphate, nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate; nucleosides: adenosine, guanosine and inosine; bases: hypoxanthine, xanthine and uric acid were determined using the high‐performance liquid chromatography method. The average value of adenylate energy charge in the group was 0.77 ± 0.10, and the mean total adenine nucleotide content (TAN) was 65.1 ± 18.3 pmol × 10 −6 spermatozoa. The mean concentrations of ATP, ADP and AMP were 43.5, 11.8 and 9.9 pmol × 10 −6 spermatozoa respectively. The concentrations of the other energetic nucleotides studied were lower. In all males studied ( n =23) with a motility rate from 0% to 95%, no statistically significant correlation between the per cent of motile sperm and ATP concentration was found ( R s =0.35), whereas the correlation between the per cent of motile sperm and ADP was statistically significant ( R s =0.60). A negative correlation was found between hypoxanthine and the per cent of motile spermatozoa ( R s =−0.44). Our results suggest that hypoxanthine is the final product of salmon spermatozoa of adenine nucleotide catabolism.