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Oxidative damages by cadmium and the protective effects of low‐molecular‐weight chitosan in the freshwater crab ( Sinopotamon yangtsekiense Bott 1967)
Author(s) -
Li Ruijin,
Zhou Yanying,
Ji Jinfang,
Wang Lan
Publication year - 2011
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2010.02646.x
Subject(s) - hepatopancreas , catalase , biology , freshwater crab , malondialdehyde , superoxide dismutase , antioxidant , nitric oxide , glutathione peroxidase , cadmium , dismutase , oxidative stress , biochemistry , oxidative phosphorylation , decapoda , fishery , endocrinology , chemistry , crustacean , organic chemistry
Cadmium (Cd) is an environmental pollutant and has posed a potential threat for the growth and survival of freshwater crabs. Low‐molecular‐weight chitosan (LMWC) may promote growth in crab culture. The present study was designed to investigate the Cd‐induced oxidative damage and the protective role of LMWC against oxidation caused by Cd 2+ in freshwater crab ( Sinopotamon yangtsekiense Bott 1967). The results showed that Cd 2+ significantly inhibited the activities of total antioxidant capacity, superoxide dismutase, catalase, glutathione peroxidase and peroxidase, while it increased malondialdehyde levels in the hepatopancreas and the gill. Moreover, Cd 2+ at the concentration tested obviously increased the protein carbonyl contents and DNA–protein crosslinks coefficients in the hepatopancreas, gill, heart and muscle tissues of S. yangtsekiense in a dose‐dependent manner. In addition, Cd 2+ induced a significant increase in the levels of nitric oxide and inducible nitric oxide synthase in the hepatopancreas, gill and muscle. The results also showed that LMWC plus Cd 2+ significantly improved antioxidant markers. The observations suggested that the severe oxidative damage in multiple crab tissues was one of the important causes of the adverse influence of Cd 2+ on S. yangtsekiense growth and indicated that LMWC could provide a protective effect against such an injury.