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Microsatellite–centromere mapping in walking catfish Clarias macrocephalus (Günther, 1864) using gynogenetic diploids
Author(s) -
Poompuang Supawadee,
Sukkorntong Chantapim
Publication year - 2011
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2010.02613.x
Subject(s) - biology , microsatellite , centromere , genetics , catfish , ploidy , genome , evolutionary biology , gene , chromosome , fish <actinopterygii> , allele , fishery
Thirty new microsatellite loci were isolated from a microsatellite‐enriched genomic library of walking catfish Clarias macrocephalus . The CA motif was the most abundant, although several other motifs were also isolated. Two gynogenetic diploid families, each consisting of a female and 50 offspring, were genotyped at 56 microsatellite loci, including 30 loci developed in the present study and 26 loci reported in the previous study. Overall, 33 anonymous microsatellites derived from genomic library and 11 EST‐linked microsatellites were mapped to their centromeres. High levels of chiasma interference were apparent in the walking catfish genome as indicated by an average frequency of second‐division segregation ( y ) of 0.643 ± 0.248. Twenty‐six loci (59%) showed a high microsatellite–centromere recombination, with a frequency >0.67, and three loci had recombination frequencies >0.9. This study demonstrated that gene–centromere mapping provided a rapid method for the expansion of the initial linkage map of walking catfish.