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Sperm motility and seminal plasma characteristics in Barbus sharpeyi (Günther, 1874)
Author(s) -
Hadi Alavi Sayyed Mohammad,
Jorfi Elham,
Hatef Azadeh,
Saheb Mortezavi Sayyed Abdul
Publication year - 2010
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2010.02600.x
Subject(s) - sperm , sperm motility , motility , distilled water , biology , flagellum , osmotic concentration , andrology , biochemistry , chemistry , chromatography , botany , microbiology and biotechnology , medicine , gene
Spermatozoa concentration, ionic composition, osmolality, glucose and total protein contents of seminal plasma and sperm motility were determined in Barbus sharpeyi (Cyprinidae, Teleosotei). Spermatozoa concentration ranged from 9.77 to 20.20 × 10 9 spermatozoa mL −1 . Osmolality (mOsmol kg −1 ) and ionic contents (mM L −1 ) of the seminal plasma were 274.5±9.0, 70.0±3.4 Na + , 28.8±0.9 K + , 101.7±3.1 Cl − , 0.9±0.1 Mg 2+ and 2.1±0.1 Ca 2+ respectively. Total protein and glucose were 5.3±0.2 g L −1 and 76.7±4.3 mM L −1 respectively. Sperm motility was initiated in a hypo‐osmotic condition, composed of either an ionic (KCl or NaCl) or a non‐ionic (sucrose) activation medium. Duration of sperm motility was very short: <2 min after activation in distilled water. Percentage of motile spermatozoa was significantly higher in an activation medium containing NaCl compared with that of distilled water. An activating medium containing NaCl or KCl higher than 150 mM or sucrose higher than 275 mM totally inhibited the activation of sperm motility. Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. Studied characteristics in the present study were compared with those of other cyprinids for understanding inter‐species differences.