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Sperm fertility of the subtropical freshwater streaked prochilod Prochilodus lineatus (Characiformes) improved after dilution and cold storage
Author(s) -
Orfão Laura H,
Maria Alexandre N,
Nascimento Ariane F,
Isaú Ziara A,
Viveiros Ana T M
Publication year - 2010
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2010.02597.x
Subject(s) - sperm , biology , extender , sperm motility , tambaqui , reproduction , characiformes , fertility , dilution , hatching , female sperm storage , zoology , ecology , botany , chemistry , fishery , population , sperm competition , demography , physics , organic chemistry , sociology , fish <actinopterygii> , polyurethane , thermodynamics
The aims of this study were to evaluate the efficiency of simple and complex extenders in prolonging the cold storage of sperm (Experiments 1 and 2) and to test the diluted‐cooled sperm in the best extender with regard to sperm quality parameters (Experiment 3) in the streaked prochilod, Prochilodus lineatus . In all the experiments, aliquots of 0.3 mL of sperm were diluted 1:10 in extenders and stored at 4–6 °C. Sperm diluted in simple extenders (NaCl and glucose solutions) yielded 0–26% sperm motility, whereas sperm diluted in complex extenders (BTS ™ , M III ™ and Androstar ™ ) yielded 62–81% sperm motility on day 4 after cold storage. When Androstar ™ was further investigated, the following was observed on day 4: 53% motility with 94 s of duration; 47% live spermatozoa; 26–61% fertility rate; and 22–60% hatching rate. The use of Androstar ™ improves the sperm fertility of the streaked prochilod during a 4‐day storage period and can therefore be used to facilitate artificial reproduction.