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Analysis of bacterial diversity in the intestine of grass carp ( Ctenopharyngodon idellus ) based on 16S rDNA gene sequences
Author(s) -
Han Shaofeng,
Liu Yuchun,
Zhou Zhigang,
He Suxu,
Cao Yanan,
Shi Pengjun,
Yao Bin,
Ringø Einar
Publication year - 2010
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2010.02543.x
Subject(s) - biology , grass carp , proteobacteria , firmicutes , bacteroidetes , 16s ribosomal rna , phragmites , diversity index , gut flora , zoology , ecology , botany , bacteria , fishery , species richness , fish <actinopterygii> , wetland , genetics , immunology
In the current study, we assessed bacterial diversity in the gut content of pond‐reared grass carp ( Ctenopharyngodon idellus ), in the associated habitat environments (pond water and sediment) and in the ingested food (commercial feed and the reed Phragmites australis ) by analysing 16S rDNA sequences from clone libraries. The highest bacterial diversity was observed in the gut content and was determined by the total number of operational taxonomic units, Shannon diversity index ( H ), Shannon equitability index ( E H ), Coverage ( C good ) and rarefaction curves calculated from the 16S rDNA gene libraries. Our data indicated that allochthonous gut microbes of grass carp were distinctively different from the corresponding environmental microbes. The pairwise similarity coefficient ( C s ) for microbe communities between gut content and ingested food was higher than for those between the gut content and habitats, indicating that the allochthonous microbiota identified in the intestines of grass carp were phylogenetically closer to those in the ingested food than to those in the habitat. Based on our study and previous research, we suggest that the digesta of grass carp harbours a microbiota phylogenetic core of Proteobacteria and Firmicutes and this observation deserves further investigations with respect to a potential pool of probiotics to grass carp.