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Growth, salinity tolerance and microsatellite analysis of the F 2 reciprocal hybrids of Oreochromis niloticus × Sarotherodon galilaeus at different salinities
Author(s) -
Yan Biao,
Wang Zhenhua
Publication year - 2010
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2010.02542.x
Subject(s) - biology , oreochromis , hybrid , salinity , zoology , tilapia , population , fishery , ecology , botany , fish <actinopterygii> , demography , sociology
Oreochromisniloticus ( O ), the sixth generation of Genetic Improvement of Farmed Tilapia, shows rapid growth but poor salt tolerance, while Sarotherodon galilaeus ( S ) exhibits opposite traits. To combine the traits, F 1 progeny was obtained through artificial fertilization. Fertile F 1 produced F 2 by natural spawning. The mean survival times, the median survival time (ST 50 ) or the survival rate of hybrids was greater than O. niloticus in a gradual or an acute salinity change. Plasma osmolarity, [Na + ] and [Cl − ] of the hybrids fluctuated in 32 g L −1 water during a 24‐h period, but eventually reached levels similar to fish in freshwater. O. niloticus ♀× S. galilaeus ♂ ( OS F 2 ) or S. galilaeus ♀× O. niloticus ♂ ( SO F 2 ) showed the fastest growth at 22.5 g L −1 , equal to about 78.2% or 69.7% of O. niloticus at 0 and 3.87 or 3.45 times that of S. galilaeus at their individual optimum growth. Growth in OS F 2 was 12% faster than SO F 2 . Microsatellite analysis showed that F 2 had more alleles, a higher polymorphism information content and greater observed and expected heterozygosity than O . or S . Population differentiation was not detected between F 1 and F 2 . All the results indicated that F 2 could be exploited for commercial production under saline conditions.