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Discovery of host defence genes in the Japanese scallop Mizuhopecten yessoensis Jay by expressed sequence tag analysis of kidney tissue
Author(s) -
Liu Weidong,
He Chongbo,
Li Wenji,
Zhou Zunchun,
Gao Xianggang,
Fu Liyuan
Publication year - 2010
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2010.02484.x
Subject(s) - scallop , biology , contig , expressed sequence tag , gene , genetics , cdna library , complementary dna , sequence analysis , computational biology , fishery , genome
The Japanese scallop Mizuhopecten yessoensis is one of the most important aquaculture mollusca in Japan and China. In the present study, a high‐quality cDNA library of the Japanese scallop was constructed from the kidney tissue. A total of 2919 expressed sequence tags longer than 100 bp were generated from this library. A cluster of 1440 unique sequences, which consisted of 258 contigs and 1182 singletons, was revealed. Based on blast searches, 882 (61.3%) genes had significant ( E ‐value <1e–5) matches to known sequences in public databases. Among them, >70 genes were involved in stress response, immunity and apoptosis. These results expanded our knowledge of the genetics and physiology of the Japanese scallop, and provided a useful resource for gene discovery for further research of this species.