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An inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi
Author(s) -
Preetha Radhakrishnan,
Jose Seena,
Prathapan Sreedharan,
Vijayan Koyadan K,
Jayaprakash Naatamai S,
Philip Rosamma,
Bright Singh Isaac S
Publication year - 2010
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2009.02436.x
Subject(s) - vibrio harveyi , penaeus monodon , biology , thin layer chromatography , pseudomonas , vibrio , phenazine , high performance liquid chromatography , chromatography , nuclear magnetic resonance spectroscopy , microbiology and biotechnology , nuclear chemistry , shrimp , bacteria , biochemistry , chemistry , organic chemistry , fishery , genetics
Persistence of the antivibrio property of the potential antagonistic probiotics, Pseudomonas MCCB 102 and 103, at different temperatures, pH and in organic solvents was studied. The antivibrio compound was extracted, purified and characterized using thin‐layer chromatography, high‐pressure liquid chromatography, liquid chromatography‐mass spectroscopy, UV–Vis and nuclear magnetic resonance spectroscopy and identified as N ‐methyl‐1‐hydroxyphenazine, a phenazine antibiotic. The toxicity of the compound was tested in Penaeus monodon haemocyte culture and the IC 50 value was found to be 1.4 ± 0.31 mg L −1 . The compound was found to be bacteriostatic at 0.5 mg L −1 . Its stability to varying temperature, pH, organic solvents, prolonged shelf‐life and vibriostatic nature point to its suitability for prophylatic aquaculture application.