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Haematologic and immunologic parameters of bullfrogs, Lithobates catesbeianus , fed probiotics
Author(s) -
Dias Danielle de Carla,
De Stéfani Marta Verardino,
Ferreira Cláudia Maris,
França Fernanda Menezes,
RanzaniPaiva Maria José Tavares,
Santos Antenor Aguiar
Publication year - 2010
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2009.02390.x
Subject(s) - biology , mean corpuscular volume , lithobates , probiotic , lactobacillus acidophilus , bifidobacterium bifidum , microbiology and biotechnology , food science , bullfrog , zoology , hematocrit , bacteria , endocrinology , genetics
The effects of two probiotics (P 1 – Lactobacillus acidophilus, Bifidobacterium bifidum and Enterococcus faecium and P 2 – Bacillus subtilis ) supplemented to commercial feed (40% crude protein) on the haematological and immunological parameters of the bullfrog Lithobates catesbeianus were studied. Two doses of each probiotic (5 and 10 g kg −1 of food) were added to the diets and fed to frogs, totalling five treatments over 112 days. Haematological analyses consisted of total and differential leucocyte counts, erythrocyte and thrombocyte counts, haematocrit, haemoglobin levels and RBC indices (mean corpuscular volume, mean corpuscular haemoglobin – and mean corpuscular haemoglobin concentration) and the immunological parameters included phagocytic capacity and phagocytic index of peritoneal phagocytes. The results showed that the probiotics did not significantly influence any of the haematological parameters measured. However, immunological assays showed that the probiotics had an immunostimulating effect. The greatest effects were seen with probiotic P 1 fed at a dose of 10 g kg −1 of diet and probiotic P 2 fed at 5 g kg −1 of diet.