Encapsulation capacity of Artemia nauplii with customized probiotics for use in the cultivation of western king prawns ( Penaeus latisulcatus Kishinouye, 1896)

The encapsulation capacity of Artemia nauplii with customized probiotics Pseudomonas synxantha and Pseudomonas aeruginosa for use in the cultivation of western king prawns ( Penaeus latisulcatus ) was investigated. Seven trials were conducted to investigate this encapsulation capacity in terms of Artemia survival and probiotic load in Artemia . Newly hatched Artemia nauplii at 250 nauplii mL −1 were fed individual probiotics at 0, 10 3 , 10 5 and 10 7 colony‐forming units (CFU) per millilitre, and mixtures of these two probiotics (10 5  CFU mL −1 ) at 30:70, 50:50 or 70:30 v/v in a medium of ozonated water (OW), tryptone soya broth (TSB), and a mixture of these media. The appropriate medium for encapsulation of probiotics by Artemia nauplii was the mixture of OW and TSB at 75:25 v/v; whereas, the use of OW or TSB alone was not effective. Artemia nauplii most effectively encapsulated the customized probiotics at 10 5  CFU mL −1 . The results indicates that the encapsulation of Artemia nauplii is optimized by using a combination of P. synxantha and P. aeruginosa at 50:50 v/v in a media mixture of OW and TSB at 75: 25 v/v. Artemia should be harvested at 48 h when survival is still high (78%) and the probiotic load in Artemia is high (3 × 10 4  CFU nauplius −1 ).