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Short‐term dietary supplementation with the microalga Parietochloris incisa enhances stress resistance in guppies Poecilia reticulata
Author(s) -
Dagar Anurag,
Zilberg Dina,
Cohen Zvi,
Boussiba Sammy,
KhozinGoldberg Inna
Publication year - 2010
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2009.02329.x
Subject(s) - poecilia , biology , carotenoid , guppy , carotene , arachidonic acid , food science , fish <actinopterygii> , zoology , botany , biochemistry , fishery , enzyme
Two trials were conducted to determine the effects of dietary enrichments with the microalga Parietochloris incisa , rich in arachidonic acid (ARA), on stress resistance in guppies Poecilia reticulata . The microalga was added to commercial diets as a neutral lipid (NL) extract and its fractions or as broken cells. Experimental diets were applied for a period of 14 days. In trial 1, commercial diets were supplemented with NL (containing 25 mg ARA and 0.11 mg β‐carotene g −1 feed), its triacylglycerol (TAG) fraction (containing 25 mg ARA g −1 feed and no β‐carotene) and the β‐carotene fraction (containing 0.11 mg carotenoid g −1 feed and minute amounts of ARA). Neutral lipid‐fed fish demonstrated the highest resistance ( P <0.05) to osmotic stress (32‐ppt NaCl), followed by fish fed with diets supplemented with TAG and β‐carotene alone, which were more resistant than control ( P <0.05). In trial 2, fish fed diets supplemented with higher levels of broken alga (26.1 mg ARA g −1 feed) were more resistant ( P <0.05) to stress as compared with fish fed lower ARA (16.3 mg g g −1 ) or an unsupplemented control diet. We suggest a dietary supplementation with broken P. incisa cells to enhance stress resistance in guppies before a stressful event.