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Delay of the egg activation process in the Black Tiger Shrimp Penaeus monodon by manipulation of magnesium levels in spawning water
Author(s) -
Pongtippatee Pattira,
Luppanakane Roengsak,
Thaweethamsewee Pinij,
Kirirat Pornpimol,
Weerachatyanukul Wattana,
Withyachumnarnkul Boonsirm
Publication year - 2010
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2009.02322.x
Subject(s) - penaeus monodon , broodstock , spawn (biology) , biology , human fertilization , shrimp , magnesium , fishery , seawater , zoology , prawn , artificial fertilization , andrology , anatomy , aquaculture , ecology , fish <actinopterygii> , chemistry , organic chemistry , medicine
The aim of this study was to determine whether magnesium (Mg 2+ ) in seawater is required for egg activation of the black tiger shrimp Penaeus monodon and whether manipulation of Mg 2+ levels can be used to delay the process and thereby synchronize egg activation. Female P. monodon broodstock were allowed to spawn in artificial seawater containing Mg 2+ at varying levels with respect to the normal (100%) level: 100%, 50%, 20% and 0%. Egg activation occurred normally at 100% Mg 2+ , incompletely at 50% and 20% Mg 2+ levels and did not occur at all with 0% Mg 2+ . The fertilization rate with 100% Mg 2+ was observed to be 83%, but fertilization failed to take place in all the other groups. The fertilization rate was restored from 0% to 76% following the 20% Mg 2+ level treatment when Mg 2+ levels returned to normal (100%) as soon as spawning was completed. This study suggests that the level of Mg 2+ in seawater plays a vital role in P. monodon egg activation, and that commencement of this process could be delayed by manipulation of the Mg 2+ level during and immediately after spawning.