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Hormone injections enhance the tolerance of land‐locked ayu spermatozoa to cryopreservation
Author(s) -
Yokoi Kenichi,
Kuwada Tomonori,
Yamane Koji,
Nishiguchi Yoshinori,
Yamamoto Shinichi,
Gouda Mitsugi,
Takii Kenji,
Ohta Hiromi
Publication year - 2009
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2009.02311.x
Subject(s) - cryopreservation , biology , motility , andrology , hormone , sperm motility , sperm , plecoglossus altivelis , endocrinology , embryo , fishery , botany , microbiology and biotechnology , fish <actinopterygii> , medicine
We evaluated the effects of maturation‐stimulating hormones on the post‐thaw motility of land‐locked form ayu ( Plecoglossus altivelis ) spermatozoa. Male ayu were administered three intraperitoneal injections of either salmon pituitary extract (SPE; 0.2 or 0.6 mg g −1 BW day −1 ) or of 17, 20β‐dihydroxy‐4‐pregnen‐3‐one (DHP; 2 or 10 μg g −1 BW day −1 ), the maturation‐inducing steroid (MIS) in ayu. Before cryopreservation, the motility of spermatozoa of the SPE‐ and DHP‐treated groups was significantly higher than that of the control group. Similarly, the comparative post‐thaw motility (presented as a percentage of the motility obtained before cryopreservation) was significantly higher in the SPE group than in the control; however, there was no significant difference between the DHP group and the control. The effect of SPE and DHP on pre‐ and post‐cryopreservation motility was not dose dependent. Our results suggest that the hormone(s) present in salmon pituitary are effective in enhancing the tolerance of ayu sperm cells to cryopreservation and that the MIS (DHP) is not involved in this process.