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A novel multiplex PCR method for detecting virulent strains of Vibrio alginolyticus
Author(s) -
Cai ShuangHu,
Lu YiShan,
Wu ZaoHe,
Jian JiChang,
Huang YuangCong
Publication year - 2009
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2009.02298.x
Subject(s) - vibrio alginolyticus , virulence , biology , multiplex polymerase chain reaction , microbiology and biotechnology , multiplex , polymerase chain reaction , variants of pcr , gene , vibrio , virology , bacteria , genetics
The bacterial strains obtained from various origins were tested with the novel primers targeting the collagenase gene, ompK gene and tox R gene to establish a multiplex polymerase chain reaction (PCR) method. These primers successfully recognized all virulent strains of Vibrio alginolyticus , but the avirulent strains were not recognized by the multiplex PCR because of lack of the collagenase and tox R genes. In a 50 μL multiplex PCR mixture, the lowest detection limit is 8.8 × 10 2 cells of virulent strains of V. alginolyticus . The multiplex PCR method was successfully developed to identify virulent strains of V. alginolyticus , and provides a rapid, sensitive, specific and reliable technology for diagnosing virulent strains of V. alginolyticus . Therefore, the novel multiplex PCR in the present paper can be useful for any laboratory working with vibriosis detection of aquatic animals.