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Promotion of callus propagation by 5‐aminolevulinic acid in a Laminaria japonica sporophyte
Author(s) -
Tabuchi Katsuhiro,
Mizuta Hiroyuki,
Yasui Hajime
Publication year - 2009
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2009.02294.x
Subject(s) - callus , sporophyte , explant culture , biology , thallus , laminaria , botany , japonica , cell division , cell , biochemistry , algae , in vitro
The effects of 5‐aminolevulinic acid (ALA) on the induction and growth of callus‐like cells in Laminaria japonica were investigated in explants obtained from basal, middle and apical portions along the sporophyte. 5‐Aminolevulinic acid treatment promoted the induction of callus‐like cells in explants obtained from all portions, and the induction rate was higher when a concentration of 50–500 mg L −1 of ALA was used. The promotion was especially remarkable in apical explants, and the induction was 10–14 times higher in the 100–500 mg L −1 range than that in the 0 mg L −1 . The cell division rate of callus‐like cells showed the highest value in the explants cultured with 500 mg L −1 of ALA for 14 days. The promotion of the cell division rate by culturing with 500 mg L −1 ALA was also observed under white, blue and red lights. The callus‐like cells, which were cultured in 500 mg L −1 of ALA for 2 months, had many clear chloroplasts. After 3 months, young thalli occurred. These results suggest that the ALA treatment is effective for stable propagation of callus‐like cells in L. japonica .