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Establishment of a novel fin cell line from Brown‐marbled grouper, Epinephelus fuscoguttatus (Forsskål), and evaluation of its viral susceptibility
Author(s) -
Wei Yunbo,
Fan Tingjun,
Jiang Guojian,
Sun Ai,
Xu Xiaohui,
Wang Jing
Publication year - 2009
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2009.02253.x
Subject(s) - biology , grouper , iridovirus , turbot , virology , cell culture , population , subculture (biology) , virus , microbiology and biotechnology , fishery , fish <actinopterygii> , genetics , demography , sociology
To lay a solid foundation of in vitro investigations of fish viral diseases, cytotechnology and cytotoxicology, a novel fin cell line from brown‐marbled grouper, Epinephelus fuscoguttatus , was established and its viral susceptibility was evaluated. The fin tissues, digested with hyaluronidase and collagenase II, were used to initiate primary culture at 24 °C by using 20% foetal bovine serum‐Dulbecco's modified Eagle medium/F12 medium, which was further supplemented with carboxymethyl–chitooligosaccharide, basic fibroblast growth factor and insulin‐like growth factor‐I. The fibroblastic fin cells grew at a steady rate during subsequent subculture and had a population doubling time of 50.6 h at passage 60. The modal diploid chromosome number was 48. A brown‐marbled grouper fin cell line (bmGF‐1) has been established and subcultured to passage 75 by now. Viral susceptibilities revealed that typical cytopathic effects of bmGF‐1 cells emerged after being infected by turbot reddish‐body iridovirus (TRBIV) or lymphocystis disease virus (LCDV). However, a large number of TRBIV and LCDV particles were also found in infected bmGF‐1 cells. All these indicate that the bmGF‐1 cell line has good susceptibility to TRBIV and LCDV, which may serve as a valuable tool for studies of cell–virus interactions and have potential applications in fish virus propagation and vaccine development.