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Pharmacokinetics and tissue residues of marbofloxacin in crucian carp ( Carassius auratus ) after oral administration
Author(s) -
Zhu Yanlei,
Tan Yeping,
Wang Chunmei,
Zhang Nan,
Liu Yunping,
Liu Lina,
Li Chunming,
Lu Xiaocong,
Cao Jiyue
Publication year - 2009
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2008.02146.x
Subject(s) - crucian carp , pharmacokinetics , cmax , volume of distribution , carassius auratus , withdrawal time , half life , oral administration , zoology , biology , residue (chemistry) , chromatography , absorption (acoustics) , chemistry , pharmacology , fish <actinopterygii> , biochemistry , fishery , genetics , colorectal cancer , colonoscopy , cancer , physics , acoustics
Pharmacokinetics and residue elimination of marbofloxacin (MBF) were studied in crucian carp ( Carassius auratus , 250±30 g) kept at two water temperatures of 15 and 25 °C. Marbofloxacin concentrations in plasma and tissues were analysed by means of high‐performance liquid chromatography using an ultraviolet detector. The limits of detection were 0.02 μg mL −1 , 0.02 μg g −1 , 0.025 μg g −1 , 0.02 μg g −1 and 0.025 μg g −1 in plasma and muscle, skin, liver and kidney respectively. Fish were administered orally at a single dosage of 10 mg kg −1 body weight in the PK group. The data were fitted to two‐compartment open models at both temperatures. At 15 °C, the absorption half‐life () and distribution half‐life ( t 1/2α ) of the drug were 0.36 and 4.48 h respectively. The corresponding values at 25 °C were 0.23 and 0.87 h respectively. The elimination half‐life ( t 1/2β ) was 50.75 h at 15 °C and 25.05 h at 25 °C. The maximum MBF concentration ( C max ) differed little between 15 (6.43 μg mL −1 ) and 25 °C (8.36 μg mL −1 ). The time to peak concentration was 1.74 h at 15 °C and 0.78 h at 25 °C. The apparent volume of distribution ( V d / F ) of MBF was estimated to be 1.36 and 0.87 L kg −1 at 15 and 25 °C respectively. The area under the concentration–time curve (AUC) was 301.80 μg mL −1 h at 15 °C and 182.80 μg mL −1 h at 25 °C. The total clearance of MBF was computed as 0.03 and 0.05 L h −1 kg −1 at 15 and 25 °C respectively. After repeated oral administration at a dosage of 10 mg kg −1 body weight per day for 3 days, the results showed that the elimination half‐lives () of MBF from all tissues at 15 °C were longer than that at 25 °C. Therefore, water temperature is an important factor to be considered when deciding a reasonable withdrawal time.