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Olfactory nerve response of masu salmon ( Oncorhynchus masou Brevoort) and rainbow trout ( O. mykiss Walbaum) to clove oil and MS‐222
Author(s) -
Yamamoto Yuzo,
Woody Carol A,
Shoji Takayuki,
Ueda Hiroshi
Publication year - 2008
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2008.01957.x
Subject(s) - rainbow trout , oncorhynchus , biology , fishery , zoology , olfactory system , fish <actinopterygii> , neuroscience
Two anaesthetics, clove oil and methane sulphonate (MS‐222), were examined for their effects on the olfactory nerve response of masu salmon ( Oncorhynchus masou Brevoort) and rainbow trout ( O. mykiss Walbaum). Exposing both species to clove oil for 3 min at concentrations of 50 and 100 mg L −1 , or for 10 min at 50 mg L −1 , did not significantly reduce their olfactory response. Directly applying clove oil anaesthesia to the olfactory epithelium significantly reduced olfactory response though after 20 min, olfactory response recovered to 70% and 52% of pre‐treatment levels in masu salmon and rainbow trout respectively. Compared with the post‐anaesthetic recovery of responses after clove oil (50 mg L −1 ), buffered MS‐222 (100 mg L −1 ) with NaHCO 3 (100 mg L −1 ), and unbuffered MS‐222 (100 mg L −1 ) treatment for 3 min, the response after MS‐222 treatment declined gradually and significantly, but not after clove oil and MS‐222+NaHCO 3 treatments. Clove oil appears to be an effective and relatively safe anaesthetic for salmonids with little long‐term impact on their olfactory response, which plays a crucial role in their life history.