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In vitro effect of the red alga Hydropuntia cornea (J. Agardh) on the respiratory burst activity of sole ( Solea senegalensis , Kaup 1858) phagocytes
Author(s) -
DíazRosales Patricia,
Felices Carmen,
Abdala Roberto,
Figueroa Félix L,
Gómez Pinchetti Juan Luis,
Moriñigo Miguel A,
Balebona Maria Carmen
Publication year - 2007
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2007.01830.x
Subject(s) - biology , respiratory burst , in vitro , euglena gracilis , incubation , algae , microbiology and biotechnology , extracellular , superoxide , euglena , biochemistry , botany , chloroplast , enzyme , gene
The stimulatory effect of different extracts from the red alga Hydropuntia cornea (formerly Gracilaria cornea ) on the respiratory burst activity of sole phagocytes was evaluated in vitro . Aqueous and ethanolic extracts and extracellular polysaccharidic fractions from algal cultures were assayed. Phagocytes, isolated from sole kidney, were incubated in vitro with different algal fractions at concentrations of 10, 5, 2 and 1 mg mL −1 . As a positive control, commercial β‐glucan from the alga Euglena gracilis was used. The results obtained indicate that an ethanolic extract from H. cornea , at 10 mg mL −1 , is capable of enhancing superoxide anion production in sole phagocytes when the cells are incubated for 30 min with the bacterium P. damselae ssp. piscicida and the extract. On the other hand, the commercial β‐glucan leads to a significant increase at highest concentration, 10 mg mL −1 , after 30 min of incubation.