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Genetic variation of the razor clam Ensis siliqua (Jeffreys, 1875) along the European coast based on random amplified polymorphic DNA markers
Author(s) -
FernándezTajes Juan,
Gaspar Miguel,
MartínezPatiño Dorotea,
McDonough Niall,
Roberts Dai,
GonzálezTizón Ana,
MartínezLage Andrés,
Méndez Josefina
Publication year - 2007
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2007.01792.x
Subject(s) - biology , rapd , mantel test , genetic distance , genetic diversity , genetic variation , genetic variability , genetic marker , genetics , veterinary medicine , zoology , genotype , population , gene , demography , sociology , medicine
Ensis siliqua is regarded as an increasingly valuable fishery resource with potential for commercial aquaculture in many European countries. The genetic variation of this razor clam was analysed by randomly amplified polymorphic DNA (RAPD) in six populations from Spain, Portugal and Ireland. Out of the 40 primers tested, five were chosen to assess genetic variation. A total of 61 RAPD loci were developed ranging in size from 400 to 2000 bp. The percentages of polymorphic loci, the allele effective number and the genetic diversity were comparable among populations, and demonstrated a high level of genetic variability. The values of Nei's genetic distance were small among the Spanish and Portuguese populations (0.051–0.065), and high between these and the Irish populations. Cluster and principal coordinate analyses supported these findings. A mantel test performed between geographic and genetic distance matrices showed a significant correlation ( r =0.84, P <0.05), suggesting an isolation by distance process.