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Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout ( Salmo trutta caspius )
Author(s) -
Hatef Azadeh,
Niksirat Hamid,
Amiri Bagher Mojazi,
Alavi Sayed Mohammad Hadi,
Karami Mahmoud
Publication year - 2007
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2007.01784.x
Subject(s) - sperm , biology , salmo , brown trout , zoology , semen , calcium , sodium , medicine , anatomy , chemistry , fishery , botany , fish <actinopterygii> , organic chemistry
Sperm density, mineral and organic composition of the seminal plasma and their physiological relationship were investigated in the Caspian brown trout ( Salmo trutta caspius ). To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26±8.84 mM sodium (Na), 33.72±2.01 mM potassium (K), 133.04±5.96 mM chlorine (Cl), 1.68±0.2 mM calcium (Ca) and 0.988±0.13 mM magnesium (Mg). The following organic components were found: total protein 0.75±0.14 mg 100 mL −1 , cholesterol 2.86±0.58 mg L −1 and glucose 3.81±1.04 mM L −1 . The mean sperm density was estimated to be 3.3 × 10 9 spermatozoa mL −1 . The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit ( R 2 =0.703, P <0.001) and sperm density and optical density ( R 2 =0.909, P <0.001), indicating that optical density can be used as a quick and accurate method of estimating sperm density. Significant relationships were also found between sperm density and Ca, Mg and total protein of seminal plasma. A significant correlation was also observed between the Ca and Mg concentrations ( R 2 =0.774, P <0.01). The following correlations were observed between mineral and organic components: total protein and Ca ( R 2 =0.462, P <0.05), total protein and Mg ( R 2 =0.518, P <0.05) and glucose and Cl ( R 2 =0.374, P <0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of sperm.