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Effect of parental stock size on F 1 genetic structure in the bay scallop, Argopecten irradians (Lamarck, 1819)
Author(s) -
Qin Yanjie,
Liu Xiao,
Zhang Haibin,
Zhang Guofan
Publication year - 2007
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2006.01644.x
Subject(s) - argopecten irradians , biology , amplified fragment length polymorphism , scallop , population , bay , hatchery , population bottleneck , effective population size , selective breeding , genetic variation , loss of heterozygosity , zoology , genetics , fishery , allele , microsatellite , genetic diversity , gene , demography , engineering , civil engineering , sociology , fish <actinopterygii>
Three F 1 families of the bay scallop, Argopecten irradians , were produced from one, two and 10 individuals. The genetic changes in these populations, which suffered recent and different levels of bottleneck, were analysed using amplified fragment length polymorphism (AFLP) techniques. In the parental stock, a total of 330 bands were detected using seven AFLP primer pairs, and 70% of the loci were polymorphic. All F 1 groups had a significantly lower proportion of polymorphic loci when compared with the initial stock, and loss of the rare loci and reduction in heterozygosity both occurred. The progeny of the larger population (i.e., N =10) exhibited a lesser amount of genetic differentiation compared with the progeny from N =2, which showed lesser differentiation than progeny from N =1. The effective population sizes ( N e ) in N =1, 2 and 10 were estimated as 1.50, 1.61 and 2.49. Based on regression analysis, we recommend that at least 340 individuals be used in hatchery populations to maintain genetic variation.