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Characterization of haemocytes of the Indian edible oyster, Crassostrea madrasensis (Preston)
Author(s) -
Ittoop Gijo,
George Kizhakkayil C,
Sanil Nandiath K,
George Rani Mary,
Sobhana Kizhakkecheruvil S,
Nisha Padmini C
Publication year - 2006
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2006.01606.x
Subject(s) - basophilic , biology , oyster , ultrastructure , cytoplasm , crassostrea , cytochemistry , staining , peroxidase , microbiology and biotechnology , anatomy , biochemistry , fishery , enzyme , pathology , medicine , genetics
The haemocytes of the Indian edible oyster Crassostrea madrasensis were characterized using light and electron microscopy. The light microscopic study was conducted by staining a monolayer of the haemocytes with Geimsa. Cells without granules and with a large nucleus occupying much of the cytoplasmic area were grouped as hyalinocytes. Those with lesser amounts of basophilic cytoplasmic granules were characterized as semigranulocytes and those with large amounts of a mixture of acidophilic and basophilic granules were termed as granulocytes. Ultrastructural studies also revealed the presence of three types of haemocytes. Scanning electron microscopic studies were used to study the spreading behaviour of the haemocytes. Cytochemical studies revealed the presence of acidphosphatase, peroxidase and prophenol oxidase in the cells.