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Evaluation of a novel pentaplex microsatellite marker system for paternity studies in Atlantic cod ( Gadus morhua L.)
Author(s) -
Wesmajervi Mette Serine,
Westgaard JonIvar,
Delghandi Madjid
Publication year - 2006
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2006.01549.x
Subject(s) - gadus , biology , atlantic cod , microsatellite , genotyping , genotype , offspring , population , allele , zoology , multiplex polymerase chain reaction , genetics , multiplex , allele frequency , fishery , fish <actinopterygii> , polymerase chain reaction , gene , demography , pregnancy , sociology
Five Atlantic cod ( Gadus morhua L.) microsatellites ( Gmo 8, Gmo 19, Gmo 35, Gmo 37 and Tch 11) were selected for parentage assignment. All markers were co‐amplified using dye‐labelled primers and sized by automated sample processing in a 3130 xl Genetic Analyser. The genotyping of samples was reproducible and alleles from all loci could be genotyped confidently (precision and accuracy above 99.7 and 99.1% respectively). The number of alleles, observed allelic and genotypic frequencies were calculated. A population database was established for fish originating from randomly selected wild‐caught Norwegian coastal‐ and North‐East Arctic cod. Combined paternity exclusion and the probability of match were 99.97 and 1.5 × 10 −9 respectively. The multiplex assay system was used successfully for parental assignment of 2336 juveniles from different mass spawning breeding tanks. Of all offspring, 91.2% were assigned to a single parental pair. In total, 523 different matings were recorded, and 69 of 70 dams and all of the 30 sires contributed to the offspring.