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Genetic variability of Channa punctatus populations using randomly amplified polymorphic DNA
Author(s) -
Nagarajan Muniyandi,
Haniffa Mohamed Abdulkather,
Gopalakrishnan Achamveetil,
Basheer Valaparambil Saidumohammad,
Muneer Abdul
Publication year - 2006
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2006.01539.x
Subject(s) - biology , rapd , genomic dna , population , genetic variation , genetic variability , veterinary medicine , genetics , zoology , dna , gene , genetic diversity , genotype , medicine , demography , sociology
Genetic variations between Channa punctatus populations collected from three rivers of south India were examined using randomly amplified polymorphic DNA (RAPD). Sixty samples from each population were collected. Genomic DNA was isolated from the blood samples. Out of the 20 primers tested, six primers were used for genetic variation studies. A total of 42 RAPD bands were amplified in C. punctatus by the six primers. The total number of bands observed ranged from 34 in the Quilon population to 37 in the Thirunelveli population. Among the three populations, the highest genetic identity (0.9231) was found between Thirunelveli and Quilon populations. The results of the present study demonstrated that Thirunelveli and Quilon populations are more related to each other than to the Coimbatore population.