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Sex organ extracts and artificial hormonal compounds as sex pheromones to attract broodfish and to induce spawning of Chinese black sleeper ( Bostrichthys sinensis Lacépède)
Author(s) -
Hong WanShu,
Chen ShiXi,
Zhang QiYong,
Zheng WeiYun
Publication year - 2006
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2006.01462.x
Subject(s) - broodstock , biology , sex pheromone , seminal vesicle , hormone , andrology , human fertilization , medicine , endocrinology , zoology , aquaculture , fishery , anatomy , fish <actinopterygii> , prostate , genetics , cancer
Ovarian, testicular and seminal vesicle extracts of mature Chinese black sleeper ( Bostrichthys sinensis Lacepede), synthetic steroids 17α‐progesterone (17α‐P), 17α,20β‐dihydroxy‐4‐pregnen‐3one (17α,20β‐P) and prostaglandins PGE 2 and PGF 2α were used in an attempt to attract broodfish and to induce spawning. Furthermore, 17α‐P, PGE 2 and PGF 2α levels were determined in extracts, and PGE 2 and PGF 2α levels were determined in holding and spawning waters. The results showed that ovarian extracts attracted more males than females to enter artificial nests, while testicular and seminal vesicle extracts attracted more females than males. The highest percentage of spawned nests were found when 17α,20β‐P and PGE 2 were administered to the nests, while the largest egg numbers and the mean highest fertilization rate were observed when PGE 2 was added. In ovarian extract, 17α‐P level was higher than levels in testicular or seminal vesicle extracts. The PGE 2 levels were significantly ( P <0.05) higher than PGF 2α in the ovarian, testicular and seminal vesicle extracts. The PGE 2 and PGF 2α levels in spawning waters were higher than those in holding waters. The PGE 2 levels were greatly significantly ( P <0.01) higher than PGF 2α in spawning and in holding waters. The results suggest that the sex organs of B. sinensis contain 17α‐P, 17α, 20β‐P, PGE 2 and PGF 2α and that 17α‐P, 17α,20β‐P and PGE 2 may act as sex pheromones in this species, attracting both male and female conspesifics to spawning sites and inducing spawning. Among the compounds tested, PGE 2 was the most effective sex pheromone for induction of spawning in B. sinensis .