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Detection of cutaneous antibodies against Flavobacterium columnare in channel catfish, Ictalurus punctatus (Rafinesque)
Author(s) -
Shoemaker Craig A,
Xu DeHai,
Shelby Richard A,
Klesius Phillip H
Publication year - 2005
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2004.01291.x
Subject(s) - catfish , ictalurus , biology , ictaluridae , antibody , microbiology and biotechnology , fish <actinopterygii> , andrology , immunology , fishery , medicine
This study was conducted to determine if antibodies against Flavobacterium columnare were present in excised skin explants of channel catfish, Ictalurus punctatus (Rafinesque) following experimental infection. An indirect ELISA was used to determine cutaneous antibody titres at day 16, day 32 post‐immersion boost and day 60 (28 days post‐intraperitoneal (IP) boost) from excised skin explants cultured in vitro . Cutaneous antibodies against F. columnare were detected in the skin explants from IP injected channel catfish and titres ranged from 16 to 128 (mean 44±standard error (SE) 12.5; N =12) from infected fish and were significantly higher ( P <0.003) than non‐infected fish (mean 2.0±0.0; N =12) at day 16 post immunization. A second group of fish was IP injected and boosted by bath immersion at day 16. Titres ranged from 4 to 16 (mean 7.6±1.5; N =10) and were significantly higher ( P <0.04) than titres detected in the control fish (mean 2.0±0.0; N =5) at day 32 post boost. A third group of fish were injected and boosted using IP injection at day 32 with titres being determined at day 60. Titres ranged from 4 to 32 (mean 12.0±3.7; N =9) and were significantly higher ( P <0.01) than titres detected in the control fish (mean 2.0±0.0; N =9). To our knowledge, this is the first description of cutaneous antibodies against F. columnare in channel catfish.