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Standardization of photometric measurement of sperm concentration from diploid and tetraploid Pacific oysters, Crassostrea gigas (Thunberg)
Author(s) -
Dong Qiaoxiang,
Eudeline Benoit,
Huang Changjiang,
Tiersch Terrence R
Publication year - 2005
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2004.01188.x
Subject(s) - sperm , biology , crassostrea , pacific oyster , ploidy , oyster , absorbance , botany , ecology , genetics , chromatography , chemistry , gene
To provide necessary standardization of procedures for cryopreservation of sperm, a spectrophotomeric method was developed to determine the sperm concentration of diploid and tetraploid Pacific oysters, Crassostrea gigas . Wavelengths of 380, 550, 581 and 780 nm were compared, and 550 and 581 nm were found to be the most sensitive and reliable. A linear relationship between sperm concentration and photometric absorbance was observed for sperm concentrations between 2 × 10 7 and 2 × 10 9 cells mL −1 . The regression equation for the standard curve at 550 nm for sperm of diploid oysters was Y =−8.528+1.165 log X . The equation for sperm of tetraploid oysters was Y =−8.844+1.236 log X . The equation at 581 nm for sperm of diploid oysters was Y =−8.07+1.104 log X . The equation at 581 nm for sperm of tetraploid oysters was Y =−8.331+1.167 log X . Comparisons derived from the standard curves at 581 nm between observed values and the predicted values indicated good agreement for sperm from diploid (coefficient of determination, r 2 =0.983) and tetraploid ( r 2 =0.980) oysters.