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Comparative efficacy of two ascorbic acid derivatives: ascorbate‐2‐polyphosphate and ascorbate‐2‐sulphate
Author(s) -
GABAUDAN J.,
VERLHAC V.,
FENSTER R.
Publication year - 1994
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.1994.tb00674.x
Subject(s) - ascorbic acid , rainbow trout , vitamin c , bioavailability , vitamin , biology , trout , zoology , food science , polyphosphate , biochemistry , phosphate , chemistry , fish <actinopterygii> , fishery , pharmacology
. Ascorbic acid (AA), an essential vitamin for flsh, is a particularly unstable compound in aquatic feeds. It has been shown that esterification in the C2 position results in good stability. There are two A A derivatives which can be produced at a reasonable price: ascorbate‐2‐poly‐ phosphate (brand name: ROVIMIX STAY‐C) and ascorbate‐2‐sulphate (AAS). Two tests were conducted with rainbow trout, Oncorhynchus my kiss (Walbaum), in order to compare the relative biological efficacy of the two sources of ascorbic acid in fish feeds. Rainbow trout (500 g average body weight) were force‐fed a single dose (20 mg A A equivalent) of ascorbate‐2‐polyphosphate (AAPP) or ascorbate‐2‐sulphate in a gelatine capsule. Ascorbic acid concentrations in the plasma were determined at various time intervals from 0 to 184h after administration. Six flsh per sampling time were used. Areas under the curves were calculated to evaluate the relative bioavailability of the two derivatives. In the second study, a 2‐month feeding trial was run with juvenile rainbow trout (initial body weight: 6g) fed diets supplemented with the two ascorbate derivatives at levels of 60, 200 or 1000mg ascorbic add equivalent/kg feed. A vitamin C‐free diet was used as the negative control. Ascorbic acid concentrations were determined in livers at day 30, and in livers and plasma at day 60. The results show that AAPP is well absorbed by rainbow trout while AAS causes only a slight rise in AA plasma levels. The feeding trial confirms the significantly higher biological efficacy of AAPP over AAS.