Open AccessExpression of recombinant phytase of B acillus subtilis E 20 in E scherichia coli HMS 174 and improving the growth performance of white shrimp, L itopenaeus vannamei , juveniles by using phytase‐pretreated soybean meal‐containing diet
Author(s) -
Cheng W.,
Chiu C.S.,
Guu Y.K.,
Tsai S.T.,
Liu C.H.
Publication year - 2013
Publication title -
aquaculture nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.941
H-Index - 79
eISSN - 1365-2095
pISSN - 1353-5773
DOI - 10.1111/j.1365-2095.2012.00946.x
Subject(s) - phytase , litopenaeus , bacillus subtilis , shrimp , biology , escherichia coli , recombinant dna , phy , food science , thermostability , enzyme , microbiology and biotechnology , biochemistry , bacteria , gene , fishery , physical layer , telecommunications , genetics , wireless , computer science
To produce a thermostable and neutral phytase (phy) of B acillus subtilis E 20 in E scherichia coli HMS 174 and evaluate its efficiency in improving growth performance. The phy C of B . subtilis E 20 was expressed in E . coli HMS 174, and then the 42‐kDa recombinant phy C was purified by N i‐ NAT and analysed by SDS – PAGE . The recombinant phy C had optimal ranges of pH of 6 ~ 7 and temperature of 50 ~ 60 °C. A thermostability analysis showed that the enzyme is a thermostable phytase, and around 33% of residual activity was detected after being incubated at 90 ~ 100 °C for 10 min. The recombinant phy C ‐pretreated soybean meal for feed preparation improved white shrimp, L itopenaeus vannamei , growth and feed efficiency. Overall, the neutral and thermostable phy C is suitable for aquafeed, and it is able to improve the nutritional utilization, resulting in enhanced shrimp growth and reduced feed costs.